Recent Publications

Protein Engineering and Selection Platform Design

Rakestraw, J.A., Aird, D., Aha, P.M., Baynes, B.M., and Lipovsek, D., "Secretion-and-Capture Cell Surface Display for Selection of Target-Binding Proteins." Prot. Eng. Des. Sel. 2011;   (SECANT® Platform paper) (Abstract, PDF)

Mena, M.A., Treynor, T. P., Mayo, S.L., and Daugherty, P.S. "Enhanced Blue Fluorescent Proteins
Isolated from Computationally Targeted Libraries." Nature Biotechnology (2006), 24:1569-1571.

Rakestraw, J.A., Sazinsky, S.L., Piatesi, A., Antipov, E., Wittrup, K.D., "Directed Evolution of a Secretory Leader for the Improved Expression of Heterologous Proteins and Full-Length Antibodies in S. cerevisiae." Biotech. and Bioeng. (2009), 103(6): 1192-1201

Rakestraw, J.A., Baskaran, A.R., and Wittrup, K.D., "A Flow Cytometric-Based Assay for the Selection of S. cerevisiae with Improved Heterologous Protein Secretion Capability." Biotechnology Progress (2006) Jul-Aug; 22(4): 1200-1208.
 
Piatesi, A., Howland, S. W., Rakestraw, J.A., Renner, C., Robson, N., Cebon, J., Ritter, G., Old, L., Wittrup, K.D., "Directed Evolution for Improved Secretion of Cancer-Testis Antigen NY-ESO-1 from Yeast." Protein Expr Purif. (2006) Aug; 48(2): 23-42.
 
Rakestraw, J.A. and Wittrup, K.D., "Contrasting Secretory Processing of Simultaneously Expressed Heterologous Proteins in Saccharomyces cerevisiae." Biotechnology and Bioengineering (2006) Apr 5; 93(5): 896-905.

Library Construction

Brian M. Baynes and William J. Blake, “Applied Cellular Engineering." Systems Bioinformatics: An Engineering Case-Based Approach,  Artech House (2007), 263-278.

Mena, M.A. and Daugherty, P.S. "Automated Design of Degenerate Codon Libraries." Prot. Engr. Des.
Sel., December (2005), 18: 559-561.

Bessette, P.H., Mena, M.A., Nguyen, A.W., Daugherty, P.S., "Construction of Designed Combinatorial
Libraries Using Gene Assembly Mutagenesis." Meth. Mol . Biol. Humana Press (2003), 231:29-37.

William Blake, Brad Chapman, Anuradha Zindal, Michael Lee, Shaun Lippow, and Brian M. Baynes.  “Pairwise selection assembly for sequence-independent construction of long-length DNA”  Nucleic Acids Research (2010), 38 (8): 2594-2602.
 
Lipovsek, D., Mena, M.A., Lippow, S.M., Basu, S., Baynes, B.M, "Library Construction for Protein
Engineering." Protein Engineering and Design, CRC Press (2009), 83-108.

Design of Enzymes with Enhanced Properties

Shaun M. Lippow, Patti M. Aha, Matthew H. Parker, William J. Blake, Brian M. Baynes, and Dasa Lipovsek.  “Creation of a Type IIs Restriction Endonuclease with a Long Recognition Sequence”  Nucleic Acids Research (2009), 39 (9): 3061-3073.  (CdnDI meganuclease construction)